Furthermore, using Nlrc4−/− enteroids as a positive control (31, 40), we quantified S.Tm-G+ infection foci in more than 70 enteroids per replicate (which represents a sufficient sampling size to obtain statistically valid results; SI Appendix, Fig. S7B), which revealed that GsdmD−/− enteroids harbored significantly more intracellular S.Tm than the WT controls. This evidence concerns the gene GSDMD and infection.