To explore the effect of AEP cleavage of DDX3X on cancer progression, we constructed glioma and breast cancer cells (U87-MG, U251-MG, and MDA-MB-231) with AEP KD as well as with tDDX3X-C rescue (AEP KD/tDDX3X-C res) and verified the lines (Figures 4A and Supplemental Figure 5A). This evidence concerns the gene DDX3X and breast cancer.