Additionally, we assessed the impact of the DDX3X N124A site on the proliferative phenotype of tumor cells by soft agar colony formation assays, grouped as the negative control (NC), DDX3X KD, DDX3X KD/DDX3X WT res, and DDX3X KD/DDX3X N124A res groups. The gene discussed is DDX3X; the disease is neoplasm.