PCR and subsequent agarose gel electrophoresis (AGE) results showed that AEP/tDDX3X-C enhanced the ES of PRDM2 exon 2, ARRB1 exon 13, and NCOR2 exon 21 in both glioma and breast cancer cells (Figure 5H and Supplemental Figure 7D). This evidence concerns the gene PRDM2 and breast cancer.