There are few studies of 3D cell culture-based global miRNA expression analysis of different types of cancer such as colorectal cancer [26], and basal and luminal breast cancer cells [27], as such, it is necessary to study the change of gene and miRNAs expression patterns in a 3D context to a better understanding of the cell signaling pathways of the HER2+ breast cancer. The gene discussed is ERBB2; the disease is breast carcinoma.