In addition, Tumorspheres are thought to better recapitulate the properties of CSCs than traditional 2-dimensional cultures.18 In our study, we obtain tumor spheres by initiating a low amount of cells, which supports the idea that each sphere originates from a single progenitor cell.19 Morever, the addition of IL-6 to the sphere-forming media has resulted in larger, and increased number of spheres, which is thought to cause a more aggressive phenotype. This evidence concerns the gene IL6 and neoplasm.