qRT-PCR and Western blot showed that BC-CM induced the expression of RGS5, TNF-α and VCAM-1 at mRNA and protein levels in VSMCs (Fig. 6A, B), representing a pro-inflammatory phenotype, different from TNF-α treatment alone, which reduced RGS5 expression in VSMCs with pro-inflammatory phenotype. This evidence concerns the gene RGS5 and breast cancer.