qRT-PCR analysis confirmed that the up-regulation profiles of mRNAs encoding proteins involved in Wnt signaling pathway including WNT3A, WNT7B, CTNNB1, MYC, SOX2 and FOSL1, in metastatic CRC cells compared to non-metastatic CRC samples (Fig. 6C). The gene discussed is CTNNB1; the disease is colorectal carcinoma.