To explore the clinical significance of the 16 stemness-related genes in the model used clinical specimens from the HPA database, HPA analysis showed that the protein levels CCBE1, CELSR3, CXCL11, GLDC, GPRC5C, PROS1, PCDHGA3, RUNDC3B were shown in Breast cancer tissues compared to normal Breast tissue and BMP4, CT83, TRO, STMN2, SCGB2A2, EGR2, IL1RL1, UGT2B11 were not found in database. Here, TRO is linked to breast cancer.