In addition, we also performed IHC staining on the tissue microarrays (TMAs) (3 normal brain tissues and 32 GBM brain tissues), and statistical analysis of IHC staining area and staining intensity product scores showed that, RPL22L1 was positively correlated with N-cadherin (R = 0.5864, P = 0.0002), RPL22L1 was positively correlated with Vimentin (R = 0.6885, P = 0.0005) (Fig. 3F). This evidence concerns the gene RPL22L1 and glioblastoma.