Further, we employed SCENIC to predict transcription patterns of tumor cells, finding a series of key TFs of HCC tumorigenesis, such as TFs in regulation of cell dedifferentiation (SPI1, HMGB3, and YBX1) and in abnormal bile acid metabolism (NR1H4, NR1I3, FOXA3 and DDIT3). This evidence concerns the gene HMGB3 and hepatocellular carcinoma.