The enrichment analysis revealed that the pathways involved in graft-versus-host disease, antigen processing and presentation (e.g., CD74 and TAPBP), leukocyte trans-endothelial migration and cell adhesion (e.g., ITGB2, ITGB7, ITGAL, CD2 and CD226) and NK-mediated cytotoxicity (e.g., GZMB, PRF1, KLRD1, ITGAL, ITGB2 and PLCG2) were most altered among the upregulated genes in CD8 TEMRA of iPD vs HC (Fig. 6j–m). The gene discussed is PRF1; the disease is graft versus host disease.