NCHF-01 can reduce the protein expression and enzyme activity of the key enzymes 6-phosphate glucose dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) in the pentose phosphate pathway (PPP).<h4>Conclusions</h4>NCHF-01 can inhibit NSCLC through oxidative stress dependent on the PPP. This evidence concerns the gene PGD and non-small cell lung carcinoma.