CENPE and colorectal adenocarcinoma: To test the effects of these mutations on the localization of CENP‐E, we generated stable colorectal adenocarcinoma DLD‐1 cell lines expressing full‐length CENP‐E (EGFP‐CENP‐EFL) or its BUBR1Mut and RZZSMut mutants (Fig 2A and B).