TP53 and malignant colon neoplasm: Interestingly, when we compared the protein levels of wtp53 and mutp53, 24 h and 72 h after transfection, our results suggested that the mutant versions of p53 are more stable than wt-p53 (Fig. 2B), but most importantly, after 72 h, β-catenin levels were not increased as a result of mut-p53-R273H expression (the same version of mut-p53 expressed in colon cancer cells) with respect to the levels found in nontransfected or empty controls.