The confocal analysis revealed that kidney organoids transfected with HIF-1α overexpressing plasmids contained the tubular parts (LTL +) and glomerular compartments (Wilm's Tumor +) which was tightly surrounded with numerous sprouting microvasculature from the periphery to the center of the organoid (Fig. 3A). This evidence concerns the gene HIF1A and Nephroblastoma.