The Top10 cell lines sensitive to SLC25A1 gene knockout were lymphoma cell lines (SUDHL10, SUDHL5, and WSUNHL), endometrial/uterine cancer cell line (JHUEM1), sarcoma cell lines (RH30 and RHJT), leukemia cell lines (ROS50 and HB1119) and breast cancer cell line (KPL1), all of which exhibited a dependency score less than − 0.85 (Fig. 10B). The gene discussed is SLC25A1; the disease is sarcoma.