UFM1 and cardiac hypertrophy: We observed that Tnfaip2 can be downregulated by ISO treatment in both Tg and NTg mice, and its expression in Tg-Ufm1 mice treated with ISO was lower than that in NTg mice (Fig. 4B), indicating that Tnfaip2 is a UFMylation-associated gene in ISO-induced cardiac hypertrophy.