Furthermore, we analyzed publicly available single-cell RNA sequencing (scRNA-seq) data (GSE131928)39, and the result showed that rRNA 2’-O-methylation-associated proteins, NOP58 and FBL, were significantly co-expressed with glioma stem cell markers, SOX2 and OLIG2 (Supplementary Fig. 8a–d). Here, OLIG2 is linked to central nervous system cancer.