Serum-induced differentiation of GSCs is associated with loss of tumorigenicity and differentiated glioma cells (DGCs) expressed low levels of the GSC marker SOX2 and BMI1 as well as high levels of astrocytes marker GFAP, which is consistent with previous studies23,24 (Supplementary Fig. 1a). The gene discussed is SOX2; the disease is central nervous system cancer.