To investigate the relationship between SOAT1 and ferroptosis in gliomas, we constructed a lentivirus-mediated RNAi targeting SOAT1 (shSOAT1, #1: 5’-TTGAACTCAAGTACCAGCCTTC-3’; #2: 5′-TAATGGTCGAATTGACATAA-3′) in glioblastoma U-87MG cells and astrocytoma U-251 cells. The gene discussed is SOAT1; the disease is glioblastoma.