Subsequently, ROC curve analysis suggested that the AUC values of WWTR1, STEAP3, SLC39A14, NOTCH2, IREB2, HIF1A, and FANCD2 were all 1.000, indicating their potential as diagnostic biomarkers for gliomas (Fig. 4A and Supplementary Fig. 1). This evidence concerns the gene NOTCH2 and glioma.