To systematically investigate regulatory factors in the IFN-γ-independent proinflammatory activation of macrophages, we carried out high-content screening (HCS) of 1220 genes associated with immune responses in IFNGR1-deficient immortalized bone marrow-derived macrophages (iBMDM, kindly provided by Dr. Feng Shao, National Institute of Biological Sciences, Beijing, China) in response to infection with vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1). Here, IFNG is linked to infection.