SMYD3 and neoplasm: To better understand the effect of SMYD3 on primary tumor growth within the prostate microenvironment and evaluate its potential role in metastasis, we next generated orthotopic xenografts by surgically implanting PC-3 Tet-on shSMYD3 cells into the anterior prostate lobe of NSG mice and administering doxycycline-treated water (Fig. 3A).