Recruitment of total ubiquitin, p97/VCP, ANKRD13A, and UBXD1 at 2 h was determined at the PV in cells pre-stimulated with IFNγ and then treated for 2 h with the E1 enzyme inhibitor, PYR41, prior to infection with Tg type II Pru. Here, UBXN6 is linked to infection.