For these tumours, using a variation on the previous image segmentation protocol (Figure 2—figure supplement 3A–D), the proportion of EpCAM+Vim+CD24+ and EpCAM+Vim+CD24- cells was quantified for each cell in over 9000 imaging fields at the tumour-stroma boundary (Figure 2—figure supplement 3E). This evidence concerns the gene VIM and neoplasm.