FOLH1 and cancer: To evaluate whetherthe 5D3-αCD3 BiTE can specifically activate T-cells in the presenceof target PSMA-positive cancer cells, isolated human PBMCs were co-culturedwith LNCaP cells (or DU145 controls) at the optimized effector-to-targetratio (E:T) of 3:142 in the medium supplementedwith 0.2 nM and 5 nM BiTE.