FOLH1 and neoplasm: Here we describe the engineering, expression, purification,and biological characterization of bispecific T-cell engagers (BiTEs)that enable targeting PSMA-positive tumor cells by host T lymphocytes.To this end, we designed the 5D3-αCD3 BiTE as a fusion of single-chainfragments of PSMA-specific 5D3 and anti-CD3 antibodies.