Along with epigenetic modifiers that were previously implicated in cancer cell fitness, such as CHD1, CHD4, DNMT3B, ELP3, SUPT16H, SUV39H2; novel hits ASH2L, RBX1 and SSRP1 were discovered as essential genes for both U373 and T98G cells suggesting common regulatory role (Supplementary Fig. 1C). Here, DNMT3B is linked to cancer.