The frequent top NMF genes ASF1B, ATAD2, BUB1, CDK1, CHAF1A, HJURP, PBK, and TOP2A (Fig. 4f) that were signature genes for the poor outcome cluster in ACC, LGG, LIHC, and LUAD (Supplementary Fig. 4 and Supplementary Data 4) were also significantly associated with poor outcome when expressed at high levels for those same cancer types (Fig. 4f). This evidence concerns the gene TOP2A and cancer.