Biomarker expression analysis by immunofluorescence revealed that biomarkers detected in CRC tumors including CK7, CDX2, β‐catenin, Ki‐67, CK20, and pan‐cytokeratin (CK‐pan) were all significantly expressed in both CRC PDTOs and CRC 3DP models (Figure 1D; Figure S2C, Supporting Information). This evidence concerns the gene KRT7 and colorectal carcinoma.