This analysis yielded a volcano plot delineating upregulated genes with functions similar to those of the upregulated genes from invasive GBM cells in hydrogels (Supplemental Figure 2G), including acetyl-CoA carboxylase 2 (ACACB) (log2FC = 0.8, P = 0.04), whose fatty acid oxidation and ferroptosis roles are phosphorylation dependent (27), and mitochondrial electron transport chain genes NDUFA4 (log2FC = 0.7, P = 0.02), NDUFB1 (log2FC = 0.7, P = 0.006), and NDUFB8, (log2FC = 1.0, P = 0.02). The gene discussed is COXFA4; the disease is glioblastoma.