For assessing the statistical significance of the coexpression of Notch3 and WNT5B in the primary and metastasis stages, a paired-sample t test was performed and demonstrated that metastatic samples showed significantly more correlation for Notch3 and WNT5B coexpression compared with the primary tumors (Figure 5F), providing further support for the importance of the Notch3/WNT5B axis in melanoma metastasis. Here, NOTCH3 is linked to melanoma.