To provide further evidence of a true lymph node metastasis of the siCtrl* cells and its dependence on WNT5B, we analyzed the presence of GFP-expressing siCtrl, siCtrl*, and siWNT5B* WM852 cells in the superficial cervical and inguinal lymph nodes by FACS 8 and 14 days after melanoma cell injection. This evidence concerns the gene WNT5B and melanoma.