Surprisingly, guides targeting the fork reversal factors (SMARCAL1, HLTF, ZRANB3, SHPRH) or components of the Fanconi Anaemia pathway were neither enriched nor depleted (Tables S6–S8), despite previous studies suggesting a genetic interaction between some of these factors and PRIMPOL in transformed cell lines (47,50). Here, HLTF is linked to Fanconi anemia.