Next, this trend was evaluated at the protein level between normal liver and HCC tissues, and as a result, the immunohistochemical data show that SAMD13 expression was higher than that compared to normal tissue (Fig. 2B).To further validate the differential expression of SAMD13, mRNA expression of SAMD13 was conducted on 1 immortalized hepatic cell line (HepaRG), 2 well-differentiated HCC cell lines (Huh7 andHepG2), and 3 poorly differentiated HCC cell lines (SK-Hep-1, SNU475, and SNU449). Here, SAMD13 is linked to hepatocellular carcinoma.