We used condition medium from CacyBP or Myd88-silenced HCC cells to polarize PMA-stimulated THP-1 monocytes to the M2 macrophages, simulating the differentiation process of the tissue-resident macrophages, and flow cytometry analysis showed that interfering with CacyBP or Myd88 did not affect the expression of CD11b and CD206, two typical markers for identifying M2 macrophage differentiation (Figure S5D-S5E), indicating that CacyBP may otherwise increase the number of TAMs in a manner by promoting the recruitment of peripheral monocytes. The gene discussed is ITGAM; the disease is hepatocellular carcinoma.