Moreover, the hub genes were validated by quantitative real time polymerase chain reaction (qRT-PCR) in animal experiments, and it was discovered that the expression of the 2 hub genes in the rat chronic ocular hypertension (COH) model was consistent with the bioinformatics analysis results of the human POAG microarray dataset, which suggested that HSPA8 and RPL15 may be involved in the occurrence and development of POAG through regulating autophagy. Here, HSPA8 is linked to open-angle glaucoma.