Herein, an MSI vs MSS CRC analyses for differential surface marker enrichment (Fig. 4e, f) showed that MSI CRC CD8+ T cells pre-dominantly exposed markers of memory phenotype (CD12781), effector memory or polyclonal TCR phenotype (CD2582), immunoregulation (PD-L183), T cell activation (CD5684), and cytokine signaling (CD12385) (Fig. 4e, g). This evidence concerns the gene CD8A and colorectal carcinoma.