CD28 and neoplasm: MSI CRC IFNG+CD8+ T cells showed 5 clusters with distinct signaling modules: stem-like progenitor (distinguished by TCF7, IL2, CD28)40, inflamed follicular-like (CXCR5, BCL6, ICOS)44, long-term immunosurveillance (EOMES, TBX21, PRDM1, KLRK1, KLRG1)45,46, tumor-reactive exhaustion (IFNG, LAG3, PDCD1, CTLA4, TIGIT, ENTPD1, HAVCR2, CXCL13, NFATC2)8,24,47 and memory differentiation (TOX, IL15RA, NFATC3, NFIL3, STAT1/2)36,40 (Fig. 2c; Supplementary Fig. S3e and Table S3).