E2F1 and metabolic dysfunction-associated steatohepatitis: These cell cycle proteins and oncogenes include cyclin dependent kinase CDK4,16,19 Gankyrin,31 Cyclin D3,20,25 and E2F1.32, 33, 34 This article demonstrates that feeding pregnant females and their subsequent offspring with HFD causes elevation of CDK4 in the livers of the offspring (Figure 4), which has been reported to be elevated in NASH patients.19