ANO1 and cystic fibrosis: In our ASL pH studies, baseline pH was surprisingly found to be more alkaline in CF-T16AKO epithelia under inflammatory conditions contrary to what was measured in CF-CTRL epithelia (Fig. 2D), suggesting that TMEM16A had an indirect role in the regulation of resting ASL pH, perhaps via regulating other CaCC, or members of the CLCA family (39).