Knockouts of various isoforms ofHIF have revealed that the increase in reporter activity uponstimulation with dimethyloxalylglycine depends on HIF-1α.By means of ChIP-qPCR in primary renal tubular cells withdifferent genotypes of rs4903064 (TT, CT, and CC), enhancedbinding of HIF-1α and HIF-1β to risk allele C has been confirmed.To elucidate the role of overexpression of DPF3 inthe development of clear-cell renal carcinoma, a knockout ofDPF3 has been performed in cells of proximal renal tubulesusing CRISPR/Cas9 technology. This evidence concerns the gene HIF1A and clear cell renal carcinoma.