4-hydroxyderricin (4-HD) treatment inhibits HCC cell growth via upregulating apoptosis-related proteins, downregulating cell cycle-related proteins, and downregulating p-AKT and p-PI3K/PI3K; however, PI3K inhibitor (LY294002) enhances the promoting effect of 4-HD on the apoptosis and cell cycle arrest of HCC cells [50]. This evidence concerns the gene AKT1 and hepatocellular carcinoma.