We further visualized the specific pathway activation among GSCL and malignant cell clusters and observed that DNA synthesis and metabolism-related pathways were enriched in GSCL, classic pathways of glioma were enriched in Class-G, inflammatory chemotaxis-related pathways were enriched in Opc-G, dopamine transmitter synthesis-related pathways were enriched in Neo-G, embryonic development-related pathways were enriched in Unidiff-G, and energy metabolism-related pathways were enriched in Ac-G (Figure 4(d) and Supplementary Table 8). The gene discussed is GSC2; the disease is glioma.