MAP1LC3B and Familial prostate cancer: Li et al. (2016a) observed that tanshinone IIA induced PC-3 cell autophagy by increasing beclin1 and LC3B expression in prostate cancer. In this process, tanshinone IIA-induced ROS was vital for autophagy start, while ROS scavenger NAC decreased beclin1, LC3B, and cleaved caspase-3 expression. Similarly, tanshinone I triggered ROS-dependent autophagy in glioblastoma, elevating LC3B and beclin-1 expression significantly in U87 MG cells (Jian et al., 2020).