With regards to this, we collected commercially available coffee beans produced from different places and measured their effects on the entry of SARS-CoV-2 by Vpp assay in the 293T-ACE2 cell line (human embryonic kidney cells transfected with ACE2 expression vector for expressing ACE2), which are sensitive to test infection efficiency through spike and ACE2 interaction. This evidence concerns the gene ACE2 and infection.