We used MDA-MB-231 breast tumor cells, which move individually in culture (Additional file 1: Fig. S8A); we found that a higher percentage of these cells achieved oriented displacements on matrices generated by TGFβ-treated EDA+ MEFs as compared to matrices generated by TGFβ-treated EDA– MEFs or non-treated MEFs lines (Fig. 6A). This evidence concerns the gene TGFB1 and breast neoplasm.