Results revealed significantly lower level of serum 25(OH)D and significantly higher levels of serum TSP1, TLR4 and TNF-α in the obese in comparison to the normal weight subjects and in the MUO and the MUNW subjects in comparison to the MHO and the MHNW subjects, respectively, indicating inflammation in obesity as well as in metabolic unhealthy status. This evidence concerns the gene THBS1 and obesity disorder.