Next, we used delong test to detect whether there were statistical differences in ROC curves between CNN1 and other hub genes, and the results showed that most hub genes were significantly different from CNN1(p < 0.05), suggesting that CNN1 might be a biomarkers of atherosclerosis (Fig. 7C,D, Supplementary Fig. S1A–M). The gene discussed is CNN1; the disease is atherosclerosis.