Statistical analyses, based on the semi-quantification of the IHC staining (ID score), indicated that LASS2 expression level was negatively correlated to the TNM stage (P = 0.013, Table 1) (TNM stages II vs. I: P = 0.011; TNM stages III–IV vs. I: P < 0.001, Fig. 2B) and tumor size (P = 0.029, Table 1), and lower LASS2 expression in CCA tumor samples compared to HCC tissue by violin plot analysis (P = 0.0052, Fig. 2C). This evidence concerns the gene CERS2 and cholangiocarcinoma.