Moreover, the CM of ASCs isolated from patients with BC (stage III) upregulated the expression levels of the regulatory molecules genes; IL-4, TGF-b1, IL-10, CCR4 and CD25 and increased the frequency of CD4+ CD25+ FOXP3+ Tregs in peripheral blood lymphocytes (PBLs) [265]. The gene discussed is TGFB1; the disease is breast cancer.