To assess the functional effects of the mis-sense mutations in the PRKAA2 gene that are associated with melanoma or non-melanoma skin cancer, we used HEK-293 cells with a double knockout of AMPK-α2 and -α1 created using the CRISPR/Cas9 method and expressed in them either wild type or mutant FLAG-tagged AMPK-α2 by transient transfection. Here, PRKAA2 is linked to skin neoplasm.