To investigate the role and mechanism of P2Y1R in astrocytes in improving cognitive behavior in AD mice, we generated a model in which the P2Y1 gene in astrocytes was inactivated by delivering the miR30-P2Y1-shRNA adenovirus vector expressing the GFAP promoter-driven into the APP/PS1 mouse brain. The gene discussed is P2RY1; the disease is Alzheimer disease.