To further explore the mechanism of SETD2 exacerbating CRC caused by SMAD4 deletion, we performed RNA sequencing analysis on IECs of Smad4Vil‐KO mice and Smad4Vil‐KO;Setd2Vil‐KO mice induced by AOM/DSS for one cycle (Figure 4A). The gene discussed is SMAD4; the disease is colorectal carcinoma.