CD28 and acute lymphoblastic leukemia: We observed Near-ETP blasts to have downregulation of stem/myeloid markers (SPINK2, C1QTNF4, HLA-DRA) and upregulation of T-cell receptor (TCR) related machinery (LAT, CD3E, CD28, LCK, PTCRA) as compared to ETP-ALL blasts, in line with commitment to the T-lineage (Supplementary Data Fig 3b).